Open AccessNap1 Links Transcription Elongation, Chromatin Assembly, and Messenger RNP Complex Biogenesis
Author(s) -
Brian C. Del Rosario,
Lucy F. Pemberton
Publication year - 2008
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.02136-07
Subject(s) - biology , chromatin , microbiology and biotechnology , chromatin remodeling , transcription (linguistics) , nucleosome , transcription factor , transcription preinitiation complex , histone , rna polymerase ii , promoter , genetics , gene , gene expression , linguistics , philosophy
Chromatin remodeling is central to the regulation of transcription elongation. We demonstrate that the conservedSaccharomyces cerevisiae histone chaperone Nap1 associates with chromatin. We show that Nap1 regulates transcription ofPHO5 , and the increase in transcript level and the higher phosphatase activity plateau observed for Δnap1 cells suggest that the net function of Nap1 is to facilitate nucleosome reassembly during transcription elongation. To further our understanding of histone chaperones in transcription elongation, we identified factors that regulate the function of Nap1 in this process. One factor investigated is an essential mRNA export and TREX complex component, Yra1. Nap1 interacts directly with Yra1 and genetically with other TREX complex components and the mRNA export factor Mex67. Additionally, we show that the recruitment of Nap1 to the coding region of actively transcribed genes is Yra1 dependent and that its recruitment to promoters is TREX complex independent. These observations suggest that Nap1 functions provide a new connection between transcription elongation, chromatin assembly, and messenger RNP complex biogenesis.