Cyclin D1 Regulates Cellular Migration through the Inhibition of Thrombospondin 1 and ROCK Signaling | Zendy

Zhiping Li | Zendy; Chenguang Wang | Zendy; Xuanmao Jiao | Zendy; Yinan Lu | Zendy; Maofu Fu | Zendy; Andrew A. Quong | Zendy; Chip Dye | Zendy; Jianguo Yang | Zendy; Maozheng Dai | Zendy; Xiaoming Ju | Zendy; Xueping Zhang | Zendy; AnPing Li | Zendy; Peter D. Burbelo | Zendy; E. Richard Stanley | Zendy; Richard G. Pestell | Zendy

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Cyclin D1 Regulates Cellular Migration through the Inhibition of Thrombospondin 1 and ROCK Signaling

Author(s) -

Zhiping Li,

Chenguang Wang,

Xuanmao Jiao,

Yinan Lu,

Maofu Fu,

Andrew A. Quong,

Chip Dye,

Jianguo Yang,

Maozheng Dai,

Xiaoming Ju,

Xueping Zhang,

AnPing Li,

Peter D. Burbelo,

E. Richard Stanley,

Richard G. Pestell

Publication year - 2006

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.02124-05

Subject(s) - cyclin d1 , biology , cyclin d , microbiology and biotechnology , cyclin b , cyclin a , cyclin a2 , cyclin , cyclin dependent kinase complex , cancer research , kinase , cell cycle , biochemistry , protein kinase a , cyclin dependent kinase 2 , gene

Cyclin D1 is overexpressed in human tumors, correlating with cellular metastasis, and is induced by activating Rho GTPases. Herein, cyclin D1-deficient mouse embryo fibroblasts (MEFs) exhibited increased adhesion and decreased motility compared with wild-type MEFs. Retroviral transduction of cyclin D1 reversed these phenotypes. Mutational analysis of cyclin D1 demonstrated that its effects on cellular adhesion and migration were independent of the pRb and p160 coactivator binding domains. Genomewide expression arrays identified a subset of genes regulated by cyclin D1, including Rho-activated kinase II (ROCKII) and thrombospondin 1 (TSP-1). cyclin D1(-/-) cells showed increased Rho GTP and ROCKII activity and signaling, with increased phosphorylation of LIM kinase, cofilin (Ser3), and myosin light chain 2 (Thr18/Ser19). Cyclin D1 repressed ROCKII and TSP-1 expression, and the migratory defect of cyclin D1(-/-) cells was reversed by ROCK inhibition or TSP-1 immunoneutralizing antibodies. cyclin E knockin to the cyclin D1(-/-) MEFs rescued the DNA synthesis defect of cyclin D1(-/-) MEFs but did not rescue either the migration defect or the abundance of ROCKII. Cyclin D1 promotes cellular motility through inhibiting ROCK signaling and repressing the metastasis suppressor TSP-1.

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