Fission Yeast RecQ Helicase Rqh1 Is Required for the Maintenance of Circular Chromosomes | Zendy

Tomoko Nanbu | Zendy; Katsunori Takahashi | Zendy; Johanne M. Murray | Zendy; Naoya Hirata | Zendy; Shinobu Ukimori | Zendy; Mai Kanke | Zendy; Hisao Masukata | Zendy; Masashi Yukawa | Zendy; Eiko Tsuchiya | Zendy; Masaru Ueno | Zendy

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Fission Yeast RecQ Helicase Rqh1 Is Required for the Maintenance of Circular Chromosomes

Author(s) -

Tomoko Nanbu,

Katsunori Takahashi,

Johanne M. Murray,

Naoya Hirata,

Shinobu Ukimori,

Mai Kanke,

Hisao Masukata,

Masashi Yukawa,

Eiko Tsuchiya,

Masaru Ueno

Publication year - 2013

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.01713-12

Subject(s) - biology , homologous recombination , rad51 , recq helicase , chromosome segregation , helicase , genetics , telomere , mutant , rad52 , microbiology and biotechnology , chromosome , homologous chromosome , centromere , flp frt recombination , recombination , genetic recombination , dna , genome instability , dna damage , gene , rna

Protection of telomeres protein 1 (Pot1) binds to single-stranded telomere overhangs and protects chromosome ends. RecQ helicases regulate homologous recombination at multiple stages, including resection, strand displacement, and resolution. Fission yeastpot1 and RecQ helicaserqh1 double mutants are synthetically lethal, but the mechanism is not fully understood. Here, we show that the synthetic lethality ofpot1 Δrqh1 Δ double mutants is due to inappropriate homologous recombination, as it is suppressed by the deletion ofrad51 + . The expression of Rad51 in thepot1 Δrqh1 Δrad51 Δ triple mutant, which has circular chromosomes, is lethal. Reduction of the expression of Rqh1 in apot1 disruptant with circular chromosomes caused chromosome missegregation, and this defect was partially suppressed by the deletion ofrad51 + . Taken together, our results suggest that Rqh1 is required for the maintenance of circular chromosomes when homologous recombination is active. Crossovers between circular monomeric chromosomes generate dimers that cannot segregate properly inEscherichia coli . We propose that Rqh1 inhibits crossovers between circular monomeric chromosomes to suppress the generation of circular dimers.

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