Transient JMJD2B-Mediated Reduction of H3K9me3 Levels Improves Reprogramming of Embryonic Stem Cells into Cloned Embryos | Zendy

Jisha Antony | Zendy; F. C. Oback | Zendy; Larry Chamley | Zendy; Björn Oback | Zendy; Götz Laible | Zendy

Open Access

Transient JMJD2B-Mediated Reduction of H3K9me3 Levels Improves Reprogramming of Embryonic Stem Cells into Cloned Embryos

Author(s) -

Jisha Antony,

F. C. Oback,

Larry Chamley,

Björn Oback,

Götz Laible

Publication year - 2012

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.01014-12

Subject(s) - biology , demethylase , reprogramming , epigenetics , histone methylation , histone , microbiology and biotechnology , histone h3 , somatic cell nuclear transfer , histone methyltransferase , epigenomics , histone code , dna methylation , embryo , genetics , embryogenesis , cell , gene expression , blastocyst , gene , nucleosome

Correct reprogramming of epigenetic marks in the donor nuclei is crucial for successful cloning by nuclear transfer. Specific epigenetic modifications, such as repressive histone lysine methylation marks, are known to be very stable and difficult to reprogram. The discovery of histone lysine demethylases has opened up opportunities to study the effects of removing repressive histone lysine methylation marks in donor cells prior to nuclear transfer. In this study, we generated mouse embryonic stem (ES) cells for the inducible expression of JMJD2B (also known as KDM4B), a demethylase that primarily removes the histone-3 lysine-9 trimethylation (H3K9me3) mark. Induction ofjmjd2b in the ES cells decreased total levels of H3K9me3 by 63%. When these cells were used for nuclear transfer, H3K9me3 levels were normalized within minutes following fusion with an enucleated oocyte. This transient reduction of H3K9me3 levels improvedin vitro development into cloned embryos by 30%.

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