Open AccessAssembly of Regulatory Factors on rRNA and Ribosomal Protein Genes in Saccharomyces cerevisiae
Author(s) -
Koji Koyamada,
Kazuhiro Ohtsuki,
Sewon Ki,
Kayo Aoyama,
Hiroyuki Takahashi,
Takehiko Kobayashi,
Katsuhiko Shirahige,
Tetsuro Kokubo
Publication year - 2007
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.00876-07
Subject(s) - biology , promoter , microbiology and biotechnology , rna polymerase ii , transcription (linguistics) , gene , saccharomyces cerevisiae , chromatin , ribosomal protein , rna polymerase , chromatin immunoprecipitation , ribosomal rna , rna , genetics , gene expression , ribosome , linguistics , philosophy
HMO1 is a high-mobility group B protein that plays a role in transcription of genes encoding rRNA and ribosomal proteins (RPGs) inSaccharomyces cerevisiae . This study uses genome-wide chromatin immunoprecipitation to study the roles of HMO1, FHL1, and RAP1 in transcription of these genes as well as other RNA polymerase II-transcribed genes in yeast. The results show that HMO1 associates with the 35S rRNA gene in an RNA polymerase I-dependent manner and that RPG promoters (138 in total) can be classified into several distinct groups based on HMO1 abundance at the promoter and the HMO1 dependence of FHL1 and/or RAP1 binding to the promoter. FHL1, a key regulator of RPGs, binds to most of the HMO1-enriched and transcriptionally HMO1-dependent RPG promoters in an HMO1-dependent manner, whereas it binds to HMO1-limited RPG promoters in an HMO1-independent manner, irrespective of whether they are transcribed in an HMO1-dependent manner. Reporter gene assays indicate that these functional properties are determined by the promoter sequence.