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The enhancer/promoter of the vitellogenin II gene (VTG ) has been extensively studied as a model system of vertebrate transcriptional control. While deletion mutagenesis andin vivo footprinting identified the transcription factor (TF) binding sites governing its tissue specificity, DNase hypersensitivity and DNA methylation studies revealed the epigenetic changes accompanying its hormone-dependent activation.

molecular and cellular biology

Ectopic Methylation of a Single Persistently Unmethylated CpG in the Promoter of the Vitellogenin Gene Abolishes Its Inducibility by Estrogen through Attenuation of Upstream Stimulating Factor Binding