Open AccessRsc4 Connects the Chromatin Remodeler RSC to RNA Polymerases
Author(s) -
Julie Soutourina,
Véronique BordasLe Floch,
Gabrielle Gendrel,
A. Flores,
Cécile Ducrot,
Hélène DumayOdelot,
Pascal Soularue,
Francisco Navarro,
Bradley R. Cairns,
Olivier Lefebvre,
Michel Werner
Publication year - 2006
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.00415-06
Subject(s) - chromatin structure remodeling (rsc) complex , biology , chromatin , computational biology , microbiology and biotechnology , genetics , chromatin remodeling , dna
RSC is an essential, multisubunit chromatin remodeling complex. We show here that the Rsc4 subunit of RSC interacted via its C terminus with Rpb5, a conserved subunit shared by all three nuclear RNA polymerases (Pol). Furthermore, the RSC complex coimmunoprecipitated with all three RNA polymerases. Mutations in the C terminus of Rsc4 conferred a thermosensitive phenotype and the loss of interaction with Rpb5. Certain thermosensitiverpb5 mutations were lethal in combination with anrsc4 mutation, supporting the physiological significance of the interaction. Pol II transcription of ca. 12% of the yeast genome was increased or decreased twofold or more in arsc4 C-terminal mutant. The transcription of the Pol III-transcribed genesSNR6 andRPR1 was also reduced, in agreement with the observed localization of RSC near many class III genes. Rsc4 C-terminal mutations did not alter the stability or assembly of the RSC complex, suggesting an impact on Rsc4 function. Strikingly, a C-terminal mutation of Rsc4 did not impair RSC recruitment to the RSC-responsive genesDUT1 andSMX3 but rather changed the chromatin accessibility of DNases to their promoter regions, suggesting that the altered transcription ofDUT1 andSMX3 was the consequence of altered chromatin remodeling.