KDM3B Is the H3K9 Demethylase Involved in Transcriptional Activation of lmo2 in Leukemia | Zendy

Ji-Young Kim | Zendy; KeeBeom Kim | Zendy; Gwang Hyeon Eom | Zendy; Nakwon Choe | Zendy; Hae Jin Kee | Zendy; Hye-Ju Son | Zendy; Si-Taek Oh | Zendy; DongWook Kim | Zendy; Jhang Ho Pak | Zendy; Hee Jo Baek | Zendy; Hoon Kook | Zendy; Yoonsoo Hahn | Zendy; Hyun Kook | Zendy; Debabrata Chakravarti | Zendy; SangBeom Seo | Zendy

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KDM3B Is the H3K9 Demethylase Involved in Transcriptional Activation of lmo2 in Leukemia

Author(s) -

Ji-Young Kim,

KeeBeom Kim,

Gwang Hyeon Eom,

Nakwon Choe,

Hae Jin Kee,

Hye-Ju Son,

Si-Taek Oh,

DongWook Kim,

Jhang Ho Pak,

Hee Jo Baek,

Hoon Kook,

Yoonsoo Hahn,

Hyun Kook,

Debabrata Chakravarti,

SangBeom Seo

Publication year - 2012

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.00133-12

Subject(s) - demethylase , biology , chromatin immunoprecipitation , acute promyelocytic leukemia , histone , histone acetyltransferase , corepressor , cancer research , ezh2 , histone methylation , microbiology and biotechnology , myeloid leukemia , retinoic acid , dna methylation , biochemistry , gene expression , repressor , promoter , gene

Histone lysine methylation and demethylation are considered critical steps in transcriptional regulation. In this report, we performed chromatin immunoprecipitation with microarray technology (ChIP-chip) analysis to examine the genome-wide occupancy of H3K9-me2 during all-trans -retinoic acid (ATRA)-induced differentiation of HL-60 promyelocytic leukemia cells. Using this approach, we found that KDM3B, which contains a JmjC domain, was downregulated during differentiation through the recruitment of a corepressor complex. Furthermore, KDM3B displayed histone H3K9-me1/2 demethylase activity and induced leukemogenic oncogenelmo2 expression via a synergistic interaction with CBP. Here, we found that KDM3B repressed leukemia cell differentiation and was upregulated in blood cells from acute lymphoblastic leukemia (ALL)-type leukemia patients. The combined results of this study provide evidence that the H3K9-me1/2 demethylase KDM3B might play a role in leukemogenesis via activation oflmo2 through interdependent actions with the histone acetyltransferase (HAT) complex containing CBP.

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