Open AccessCellular Factors Required for Lassa Virus Budding
Author(s) -
Shuzo Urata,
Takeshi Noda,
Yoshihiro Kawaoka,
Hideyoshi Yokosawa,
Jiro Yasuda
Publication year - 2006
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.80.8.4191-4195.2006
Subject(s) - lassa virus , biology , budding , lassa fever , virology , arenavirus , virus , tsg101 , vp40 , microbiology and biotechnology , viral replication , genetics , microrna , gene , lymphocytic choriomeningitis , cytotoxic t cell , microvesicles , in vitro
It is known that Lassa virus Z protein is sufficient for the release of virus-like particles (VLPs) and that it has two L domains, PTAP and PPPY, in its C terminus. However, little is known about the cellular factor for Lassa virus budding. We examined which cellular factors are used in Lassa virus Z budding. We demonstrated that Lassa Z protein efficiently produces VLPs and uses cellular factors, Vps4A, Vps4B, and Tsg101, in budding, suggesting that Lassa virus budding uses the multivesicular body pathway functionally. Our data may provide a clue to develop an effective antiviral strategy for Lassa virus.