Open AccessIdentification of a New Quaternary Neutralizing Epitope on Human Immunodeficiency Virus Type 1 Virus Particles
Author(s) -
Miroslaw K. Gorny,
Leonidas Stamatatos,
Barbara Volsky,
Kathy Revesz,
Constance Williams,
Xiaohong Wang,
Sandra Cohen,
Robert Staudinger,
Susan ZollaPazner
Publication year - 2005
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.79.8.5232-5237.2005
Subject(s) - epitope , biology , monoclonal antibody , virology , neutralization , virus , epitope mapping , linear epitope , antibody , immunology
The selection of human monoclonal antibodies (MAbs) specific for human immunodeficiency virus (HIV) type 1 by binding assays may fail to identify Abs to quaternary epitopes on the intact virions. The HIV neutralization assay was used for the selection of human MAb 2909, which potently neutralizes SF162 and recognizes an epitope on the virus surface but not on soluble proteins. Three regions of gp120, the V2 and V3 loops and the CD4 binding domain, contribute to the epitope recognized by MAb 2909. The existence of such a unique MAb, which defines a complex epitope formed by a quaternary structure, suggests that there may be other new neutralizing HIV epitopes to target with vaccines.