Open AccessEntry of Pseudorabies Virus: an Immunogold-Labeling Study
Author(s) -
Harald Granzow,
Barbara G. Klupp,
Thomas C. Mettenleiter
Publication year - 2005
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.79.5.3200-3205.2005
Subject(s) - viral tegument , biology , capsid , immunoelectron microscopy , immunogold labelling , microbiology and biotechnology , pseudorabies , nuclear pore , viral envelope , lipid bilayer fusion , virology , microtubule , cytoplasm , virus , ultrastructure , anatomy , antibody , immunology
Herpesviruses infect cells by fusion of the viral envelope with cellular membranes, primarily the plasma membrane. During this process structural components of the mature virion are lost from the invading nucleocapsid, which then travels along microtubules to the nuclear pore. We examined the penetration process by immunoelectron microscopy and analyzed which of the major tegument proteins remained associated with the incoming capsid. We show that the UL36, UL37, and US3 proteins were present at intracytoplasmic capsids after penetration, whereas the UL11, UL47, UL48, and UL49 tegument proteins were lost. Thus, the three capsid-associated tegument proteins are prime candidates for viral proteins that interact with cellular motor proteins for transport of nucleocapsids to the nucleus.