Open AccessReversion In Vivo after Inoculation of a Molecular Proviral DNA Clone of Simian Immunodeficiency Virus with a Cytotoxic-T-Lymphocyte Escape Mutation
Author(s) -
Masahiro Kobayashi,
Harukazu Igarashi,
Akihiro Takeda,
Mamoru Kato,
Tetsuro Matano
Publication year - 2005
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.79.17.11529-11532.2005
Subject(s) - biology , ctl* , virology , simian immunodeficiency virus , clone (java method) , reversion , virus , cytotoxic t cell , provirus , mutant , mutation , viral replication , dna , genetics , in vitro , phenotype , gene , genome
Vaccine-based control of the replication of a simian immunodeficiency virus (SIV), SIVmac239, in macaques has recently been shown. In the process of the control, a mutant virus escaping from epitope-specific cytotoxic-T-lymphocyte (CTL) responses was rapidly selected and contained. In this study, we show that the wild-type virus appeared and became predominant in the absence of the epitope-specific CTL after inoculation of naive macaques with a molecular clone DNA of the CTL escape mutant SIV. This is the first report describing reversion in vivo from an inoculated, molecular proviral DNA clone of immunodeficiency virus with a CTL escape mutation.