Open AccessDetection and Analysis of Six Lizard Adenoviruses by Consensus Primer PCR Provides Further Evidence of a Reptilian Origin for the Atadenoviruses
Author(s) -
James F. X. Wellehan,
April J. Johnson,
Balázs Harrach,
Mária Benkö,
Allan P. Pessier,
Calvin M. Johnson,
Michael M. Garner,
April L. Childress,
Elliott R. Jacobson
Publication year - 2004
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.78.23.13366-13369.2004
Subject(s) - biology , gecko , lizard , skink , primer (cosmetics) , phylogenetic tree , virology , sequence analysis , polymerase chain reaction , genetics , gene , zoology , chemistry , organic chemistry
A consensus nested-PCR method was designed for investigation of the DNA polymerase gene of adenoviruses. Gene fragments were amplified and sequenced from six novel adenoviruses from seven lizard species, including four species from which adenoviruses had not previously been reported. Host species included Gila monster, leopard gecko, fat-tail gecko, blue-tongued skink, Tokay gecko, bearded dragon, and mountain chameleon. This is the first sequence information from lizard adenoviruses. Phylogenetic analysis indicated that these viruses belong to the genus Atadenovirus, supporting the reptilian origin of atadenoviruses. This PCR method may be useful for obtaining templates for initial sequencing of novel adenoviruses.