Open AccessTreatment of Human Immunodeficiency Virus Type 1 Virions Depleted of Cyclophilin A by Natural Endogenous Reverse Transcription Restores Infectivity
Author(s) -
Mahfuz Khan,
Minerva T. Garcia-Barrio,
Michael D. Powell
Publication year - 2003
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.77.7.4431-4434.2003
Subject(s) - cypa , infectivity , cyclophilin a , biology , capsid , virology , endogeny , mutant , virus , cyclophilin , reverse transcriptase , transcription (linguistics) , cis trans isomerases , peptidylprolyl isomerase , microbiology and biotechnology , rna , gene , biochemistry , isomerase , linguistics , philosophy
We have previously shown that virions with nef deleted can be restored to wild-type infectivity by treatment to induce natural endogenous reverse transcription (NERT). Since Nef and cyclophilin A (CyPA) appear to act in similar ways on postentry events, we determined whether NERT treatment would restore infectivity to virions depleted of CyPA. Our results show that the infectivity of virions depleted of CyPA by treatment with cyclosporine A could be restored by NERT treatment, while mutants in the CyPA binding loop of capsid could only be partially restored. These results suggest that CyPA is involved in some aspect of the uncoating process.