Open AccessA Bipartite Late-Budding Domain in Human Immunodeficiency Virus Type 1
Author(s) -
Juan Martín-Serrano,
Paul D. Bieniasz
Publication year - 2003
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.77.22.12373-12377.2003
Subject(s) - biology , budding , tsg101 , complementation , virology , human immunodeficiency virus (hiv) , virus , genetics , gene , microvesicles , phenotype , microrna
Human immunodeficiency virus type 1 (HIV-1) encodes a PTAP motif within the p6 domain of Gag that recruits Tsg101 and associated factors to facilitate virion budding. In this study, we use trans-complementation assays to demonstrate that the PTAP motif acts synergistically with additional p6 sequences to mediate the formation of infectious extracellular HIV-1 virions. These studies suggest that Tsg101 recruitment is necessary but not sufficient to account for late-budding activity exhibited by HIV-1 p6.