Open AccessDifferential Hyperacetylation of Histones H3 and H4 upon Promoter-Specific Recruitment of EBNA2 in Epstein-Barr Virus Chromatin
Author(s) -
Nathalie Alazard-Dany,
Henri Gruffat,
Edwige Hiriart,
Alain Sergeant,
Évelyne Manet
Publication year - 2003
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.77.14.8166-8172.2003
Subject(s) - biology , chromatin immunoprecipitation , promoter , chromatin , histone , histone h4 , histone h3 , chia pet , microbiology and biotechnology , chip on chip , dna , gene , chromatin remodeling , genetics , gene expression
Epstein-Barr virus nuclear antigen 2 (EBNA2) is a transcriptional activator involved in the immortalization of B lymphocytes by the virus. EBNA2 is targeted to the promoters of its responsive genes, via interaction with cellular DNA-binding proteins. Using chromatin immunoprecipitation assays, we show for the first time the conditional recruitment of EBNA2 on two specific viral promoters in vivo and demonstrate a correlation between this recruitment and a local change in the acetylation of histones H3 and H4, which is promoter dependent.