Open AccessExtensive Cross-Reactivity of CD4+Adenovirus-Specific T Cells: Implications for Immunotherapy and Gene Therapy
Author(s) -
Bianca Heemskerk,
Louise A. VeltropDuits,
Tamara van Vreeswijk,
M. M. ten Dam,
Sebastiaan Heidt,
René E. M. Toes,
Maarten J.D. van Tol,
Marco W. Schilham
Publication year - 2003
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.77.11.6562-6566.2003
Subject(s) - biology , adenoviridae , peripheral blood mononuclear cell , immunotherapy , virology , immunology , genetic enhancement , t cell , cytotoxic t cell , microbiology and biotechnology , gene , immune system , in vitro , genetics
Adenovirus (Ad)-specific T-cell responses in healthy adult donors were investigated. Ad5, inactivated by methylene blue plus visible light, induced proliferation and gamma interferon (IFN-gamma) production in peripheral blood mononuclear cells of the majority of donors. Responding T cells were CD4(+) and produced IFN-gamma upon restimulation with infectious Ad5 and Ads of different subgroups. T-cell clones showed distinct cross-reactivity patterns recognizing Ad serotypes from either one subgroup (C), two subgroups (B and C), or three subgroups (A, B, and C). This cross-reactivity of Ad-specific T cells has relevance both for Ad-based gene therapy protocols, as well as for the feasibility of T-cell-mediated adoptive immunotherapy in recipients of an allogeneic stem cell transplantation.