Open AccessBaculovirus Infection Raises the Level of TATA-Binding Protein That Colocalizes with Viral DNA Replication Sites
Author(s) -
Ilja Quadt,
Daniela Mainz,
R. M. W. Mans,
Andreas Kremer,
Dagmar Knebel-Mörsdorf
Publication year - 2002
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.76.21.11123-11127.2002
Subject(s) - biology , virology , transcription (linguistics) , viral replication , microbiology and biotechnology , autographa californica , tata binding protein , viral structural protein , dna replication , viral entry , polymerase , dna , virus , transcription factor , dna binding protein , gene , genetics , spodoptera , philosophy , linguistics , recombinant dna
During the infection cycle of Autographa californica multicapsid nuclear polyhedrosis virus, the TATA-binding protein (TBP) of the insect host cell likely participates in early viral transcription, which is mediated by the host RNA polymerase II. However, the role of TBP in late and very late viral transcription, which is accomplished by an alpha-amanitin-resistant RNA polymerase, is unclear. We observed a dramatic increase of TBP protein during the late phases of infection. TBP mRNA levels, however, were not coordinately increased. Indirect-immunofluorescence studies revealed a nuclear redistribution of TBP during infection. After labeling of viral replication centers with bromodeoxyuridine (BrdU), costaining of TBP and BrdU showed that TBP localized to viral DNA replication centers. These results suggest a putative role of TBP during late viral transcription, which may occur in close proximity to viral DNA replication.