Open AccessInteraction between Long-Distance Transport Factor and Hsp70-Related Movement Protein of Beet Yellows Virus
Author(s) -
Alexey I. Prokhnevsky,
Valera V. Peremyslov,
Alberto J. Napuli,
Valerian V. Dolja
Publication year - 2002
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.76.21.11003-11011.2002
Subject(s) - biology , movement protein , bimolecular fluorescence complementation , microbiology and biotechnology , phloem , transport protein , two hybrid screening , virus , plant virus , immunoprecipitation , yeast , virology , gene , genetics , coat protein , botany , rna
Systemic spread of viruses in plants involves local movement from cell to cell and long-distance transport through the vascular system. The cell-to-cell movement of the Beet yellows virus (BYV) is mediated by a movement protein that is an Hsp70 homolog (Hsp70h). This protein is required for the assembly of movement-competent virions that incorporate Hsp70h. By using the yeast two-hybrid system, in vitro coimmunoprecipitation, and in planta coexpression approaches, we show here that the Hsp70h interacts with a 20-kDa BYV protein (p20). We further demonstrate that p20 is associated with the virions presumably via binding to Hsp70h. Genetic and immunochemical analyses indicate that p20 is dispensable for assembly and cell-to-cell movement of BYV but is required for the long-distance transport of virus through the phloem. These results reveal a novel activity for the Hsp70h that provides a molecular link between the local and systemic spread of a plant virus by docking a long-distance transport factor to virions.