Open AccessProtective Antiviral Immune Responses to Pseudorabies Virus Induced by DNA Vaccination Using Dimethyldioctadecylammonium Bromide as an Adjuvant
Author(s) -
E.M.A. van Rooij,
Harrie L. Glansbeek,
Luuk Hilgers,
Eddie G. te Lintelo,
Y.E. de Visser,
W.J.A. Boersma,
Bart L. Haagmans,
A.T.J. Bianchi
Publication year - 2002
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.76.20.10540-10545.2002
Subject(s) - pseudorabies , dna vaccination , biology , virology , adjuvant , vaccination , immune system , virus , plasmid , microbiology and biotechnology , immunity , interferon , dna , immunology , immunization , genetics
To enhance the efficacy of a DNA vaccine against pseudorabies virus (PRV), we evaluated the adjuvant properties of plasmids coding for gamma interferon or interleukin-12, of CpG immunostimulatory motifs, and of the conventional adjuvants dimethyldioctadecylammonium bromide in water (DDA) and sulfolipo-cyclodextrin in squalene in water. We demonstrate that a DNA vaccine combined with DDA, but not with the other adjuvants, induced significantly stronger immune responses than plasmid vaccination alone. Moreover, pigs vaccinated in the presence of DDA were protected against clinical disease and shed significantly less PRV after challenge infection. This is the first study to demonstrate that DDA, a conventional adjuvant, enhances DNA vaccine-induced antiviral immunity.