Open AccessMatrix Metalloproteinase Expression Correlates with Virulence following Neurotropic Mouse Hepatitis Virus Infection
Author(s) -
Jiehao Zhou,
Stephen A. Stohlman,
Roscoe Atkinson,
David R. Hinton,
Norman W. Marten
Publication year - 2002
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.76.15.7374-7384.2002
Subject(s) - biology , virulence , matrix metalloproteinase , chemokine , virus , virology , mouse hepatitis virus , infiltration (hvac) , viral replication , central nervous system , immunology , gene expression , inflammation , microbiology and biotechnology , gene , pathology , infectious disease (medical specialty) , disease , medicine , biochemistry , physics , covid-19 , thermodynamics , neuroscience
The relationship(s) between viral virulence and matrix metalloproteinase (MMP) expression in the central nervous system (CNS) of mice undergoing lethal and sublethal infections with neurotropic mouse hepatitis virus was investigated. Lethal infection induced increased levels of MMP-3 and MMP-12 mRNAs as well as that of tissue inhibitor of matrix metalloproteinases 1 (TIMP-1) compared to sublethal infection. Increased induction of MMP, TIMP, and chemokine expression correlated with increased virus replication but not with inflammatory cell infiltration. Infection of immunosuppressed mice suggested that expression of most MMP, TIMP, and chemokine mRNA was induced primarily in CNS-resident cells. By contrast, MMP-9 protein activity was associated with the infiltration of neutrophils into the CNS. These data indicate an association between the magnitude of inflammatory gene expression within the CNS and viral virulence.