Open AccessPrior Vaccination Increases the Epitopic Breadth of the Cytotoxic T-Lymphocyte Response That Evolves in Rhesus Monkeys following a Simian-Human Immunodeficiency Virus Infection
Author(s) -
Sampa Santra,
Dan H. Barouch,
Marcelo J. Kuroda,
Jörn E. Schmitz,
Georgia R. Krivulka,
Kristin Beaudry,
Carol I. Lord,
Michelle A. Lifton,
Linda S. Wyatt,
Bernard Moss,
Vanessa M. Hirsch,
Norman L. Letvin
Publication year - 2002
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.76.12.6376-6381.2002
Subject(s) - ctl* , biology , virology , vaccinia , simian immunodeficiency virus , cytotoxic t cell , virus , immunology , modified vaccinia ankara , vaccination , poxviridae , immune system , recombinant dna , cd8 , genetics , in vitro , gene
Although recent evidence has confirmed the importance of cytotoxic T-lymphocyte (CTL) responses in controlling human immunodeficiency virus type 1 and simian immunodeficiency virus replication, the relevance of the epitopic breadth of those CTL responses remains unexplored. In the present study, we sought to determine whether vaccination can expand CTL populations which recognize a repertoire of viral epitopes that is greater than is typically generated in the course of a viral infection. We demonstrate that potent secondary CTL responses to subdominant epitopes are rapidly generated following a pathogenic simian-human immunodeficiency virus challenge of rhesus monkeys vaccinated with plasmid DNA or recombinant modified vaccinia virus Ankara vaccines. These data indicate that prior vaccination can increase the breadth of the CTL response that evolves after an AIDS virus infection.