Open AccessRecombinant Newcastle Disease Virus as a Vaccine Vector
Author(s) -
Takaaki Nakaya,
Jérôme Cros,
ManSeong Park,
Yurie Nakaya,
Hongyong Zheng,
Ana María Alfaro de Prado Sagrera,
Enrique Villar,
Adolfo Garcı́a-Sastre,
Peter Palese
Publication year - 2001
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.75.23.11868-11873.2001
Subject(s) - virology , biology , virus , newcastle disease , recombinant dna , hemagglutinin (influenza) , reverse genetics , recombinant virus , vector (molecular biology) , influenza a virus , orthomyxoviridae , gene , genetics , genome
A complete cDNA clone of the Newcastle disease virus (NDV) vaccine strain Hitchner B1 was constructed, and infectious recombinant virus expressing an influenza virus hemagglutinin was generated by reverse genetics. The rescued virus induces a strong humoral antibody response against influenza virus and provides complete protection against a lethal dose of influenza virus challenge in mice, demonstrating the potential of recombinant NDV as a vaccine vector.