Open AccessNovel cis -Acting Replication Element in the Adeno-Associated Virus Type 2 Genome Is Involved in Amplification of Integrated rep-cap Sequences
Author(s) -
Pascale y,
Jacques Tessier,
Gilliane Chadeuf,
Peter Ward,
Aurélie Giraud,
Marc Dugast,
R. J. Linden,
Philippe Moullier,
Anna Salvetti
Publication year - 2001
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.75.20.9991-9994.2001
Subject(s) - biology , plasmid , adeno associated virus , genome , gene , viral replication , virology , virus , genetics , microbiology and biotechnology , transfection , rolling circle replication , origin of replication , dna replication , recombinant dna , vector (molecular biology)
This study identifies a region of the adeno-associated virus type 2 (AAV-2)rep gene (nucleotides 190 to 540 of wild-type AAV-2) as acis -acting Rep-dependent element able to promote the replication of transiently transfected plasmids. This viral element is also shown to be involved in the amplification of integrated sequences in the presence of adenovirus and Rep proteins.