Open AccessRecombinant Expression of Lymphocytic Choriomeningitis Virus Strain WE Glycoproteins: a Single Amino Acid Makes the Difference
Author(s) -
Winfried Beyer,
Hrvoje Miletić,
Wolfram Ostertag,
Dorotheé von Laer
Publication year - 2001
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.75.2.1061-1064.2001
Subject(s) - lymphocytic choriomeningitis , biology , glycoprotein , virology , arenavirus , complementary dna , virus , microbiology and biotechnology , recombinant dna , togaviridae , gene , genetics , antigen , cd8
Cytoplasmic vector systems are generally used for expression of lymphocytic choriomeningitis virus (LCMV) proteins. However, we achieved high levels of cell surface glycoproteins using a standard nuclear expression plasmid. Expression was independent of other LCMV proteins but was blocked by a missense mutation within the original LCMV(WE) glycoprotein cDNA.