Open AccessRetroviral Splicing Suppressor Requires Three Nonconsensus Uridines in a 5′ Splice Site-Like Sequence
Author(s) -
Robert E. Paca,
Catherine S. Hibbert,
Christopher T. O'Sullivan,
Karen Beemon
Publication year - 2001
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.75.16.7763-7768.2001
Subject(s) - rna splicing , rous sarcoma virus , biology , rna , splice , splice site mutation , sequence (biology) , exonic splicing enhancer , small nuclear rna , consensus sequence , intron , alternative splicing , virology , genetics , microbiology and biotechnology , virus , messenger rna , peptide sequence , gene , non coding rna
Rous sarcoma virus RNA contains a negative regulator of splicing (NRS) element that aids in maintenance of unspliced RNA. The NRS binds U1 snRNA at a sequence that deviates from the 5' splice site consensus by substitution of U's for A's at three positions: -2, +3, and +4. All three of these U's are important for NRS-mediated splicing suppression. Substitution of a single nonconsensus C or G at any of these sites diminished NRS activity, whereas substitution of a single A generated a preferred 5' splice site within the NRS.