Open AccessImmortalization of T Lymphocytes by Human T-Cell Leukemia Virus Type 1 Is Independent of the Tax-CBP/p300 Interaction
Author(s) -
Michael D. Robek,
Lee Ratner
Publication year - 2000
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.74.24.11988-11992.2000
Subject(s) - biology , human t lymphotropic virus 1 , phosphoprotein , creb binding protein , transcription (linguistics) , t cell , clone (java method) , virology , transcription factor , viral replication , virus , microbiology and biotechnology , t cell leukemia , creb , genetics , phosphorylation , gene , immune system , linguistics , philosophy
The human T-cell leukemia virus type 1 (HTLV-1) Tax oncoprotein is a 40-kDa nuclear phosphoprotein which functions in the viral replication cycle as a transcriptionaltrans -activator of the viral long terminal repeat. Tax interacts with a variety of different transcription factors, including the CREB binding protein (CBP)/p300 family of transcriptional accessory proteins. We demonstrate that a Tax mutant defective for the CBP/p300 interaction retains the capacity to immortalize primary human T lymphocytes when it is expressed from a functional molecular clone of HTLV-1. Thus, immortalization of HTLV-1-infected cells appears to be independent of Tax-induced alterations in CBP/p300 function.