Characterization of Bluetongue Virus Ribonucleic Acid | Zendy

D. W. Verwoerd | Zendy; H. Louw | Zendy; R. A. Oellermann | Zendy

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Characterization of Bluetongue Virus Ribonucleic Acid

Author(s) -

D. W. Verwoerd,

H. Louw,

R. A. Oellermann

Publication year - 1970

Publication title -

journal of virology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.617

H-Index - 292

eISSN - 1070-6321

pISSN - 0022-538X

DOI - 10.1128/jvi.5.1.1-7.1970

Subject(s) - biology , rna , nucleic acid , virus , orbivirus , microbiology and biotechnology , sucrose gradient , electrophoresis , denaturation (fissile materials) , polyacrylamide gel electrophoresis , nucleic acid denaturation , biochemistry , reoviridae , virology , chemistry , enzyme , gene , nuclear chemistry , rotavirus

An improved purification procedure yielded bluetongue virus free from any single-stranded ribonucleic acid (RNA) component. Double-stranded RNA obtained from purified virus or isolated from infected cells was fractionated into 5 components by means of sucrose gradient sedimentation analysis, and into 10 components by electrophoresis on polyacrylamide gels. The size of these components vary from 0.5 × 106 to 2.8 × 106 daltons, with a total molecular weight estimate of about 1.5 × 107 for the viral nucleic acid. The denaturation of the genome and separation of the resulting fragments are also discussed.

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