Separation of DNA Sequences Complementary to the RNA of Avian Myeloblastosis Virus from Chicken DNA by Alkaline Cesium Chloride Density Sedimentation | Zendy

M. Shoyab | Zendy; M A Baluda | Zendy

Open Access

Separation of DNA Sequences Complementary to the RNA of Avian Myeloblastosis Virus from Chicken DNA by Alkaline Cesium Chloride Density Sedimentation

Author(s) -

M. Shoyab,

M A Baluda

Publication year - 1973

Publication title -

journal of virology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.617

H-Index - 292

eISSN - 1070-6321

pISSN - 0022-538X

DOI - 10.1128/jvi.12.3.534-537.1973

Subject(s) - biology , dna , rna , guanine , cytosine , microbiology and biotechnology , genome , nucleic acid thermodynamics , virology , biochemistry , nucleotide , gene

Density gradient sedimentation in alkaline cesium chloride of DNA from normal chicken embryos or leukemic myeloblasts fragmented to a size of 13S revealed that the DNA sequences complementary to 70S avian myeloblastosis virus RNA sedimented in the high guanine plus cytosine region ahead of the main peak of cellular DNA. When the DNA was fragmented into pieces of 6.6S there was a broader distribution of the DNA sequences complementary to the viral RNA. This technique could be employed as a step towards the isolation of DNA copies of the entire viral RNA genome from the mass of host cellular DNA.

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