Properties of Somatic Cell Hybrids Between Mouse Cells and Simian Virus 40-Transformed Rat Cells | Zendy

J. van der Noordaa | Zendy; A van Bentum-van Haagen | Zendy; J. M. M. Walboomers | Zendy; H. van Someren | Zendy

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Properties of Somatic Cell Hybrids Between Mouse Cells and Simian Virus 40-Transformed Rat Cells

Author(s) -

J. van der Noordaa,

A van Bentum-van Haagen,

J. M. M. Walboomers,

H. van Someren

Publication year - 1972

Publication title -

journal of virology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.617

H-Index - 292

eISSN - 1070-6321

pISSN - 0022-538X

DOI - 10.1128/jvi.10.1.67-72.1972

Subject(s) - biology , hybrid , agglutination (biology) , virus , somatic cell , virology , concanavalin a , chromosome , microbiology and biotechnology , simian , cell culture , antigen , genetics , gene , in vitro , botany

Hybrids between mouse cells and simian virus 40 (SV40)-transformed rat cells were made, and their properties and chromosome constitution were investigated over many generations. Their hybrid nature was confirmed by enzyme studies. During a period of 1 year a loss of 10 to 20% of the total number of chromosomes was observed. The SV40 tumor antigen was present and remained present in the hybrids. The parental and hybrid cells were studied for agglutination with concanavalin A, for growth in soft agar, and for serum requirement. These growth and surface characteristics of the transformed cells appeared in the hybrids.

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