Open AccessHIV-1 Neutralization Coverage Is Improved by Combining Monoclonal Antibodies That Target Independent Epitopes
Author(s) -
Nicole A. DoriaRose,
Mark K. Louder,
Zhongjia Yang,
Sijy O’Dell,
Martha Nason,
Stephen D. Schmidt,
Krisha McKee,
Michael S. Seaman,
Robert T. Bailer,
John R. Mascola
Publication year - 2012
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.06745-11
Subject(s) - neutralization , epitope , monoclonal antibody , virology , biology , glycoprotein , viral envelope , antibody , neutralizing antibody , human immunodeficiency virus (hiv) , microbiology and biotechnology , immunology , virus
HIV-1 neutralizing monoclonal antibodies (MAbs) define key targets for vaccine development and are being considered for passive prevention of infection. We analyzed the interaction of MAbs to two independent epitopes on the viral envelope glycoprotein. Potently neutralizing MAbs to the CD4 binding site and V1V2 region displayed noin vitro cross-competition and displayed additive, though not synergistic, neutralization activity. Predicted neutralization coverage of a combination of two MAbs reached 97% on a 208-isolate panel.