Open AccessRecombinant Influenza A Viruses with Enhanced Levels of PB1 and PA Viral Protein Expression
Author(s) -
Alan BelichaVillanueva,
Juan R. Rodríguez-Madoz,
Jad Maamary,
Alina Baum,
Dabeiba BernalRubio,
Maria Minguito de la Escalera,
Ana FernandezSesma,
Adolfo Garcı́a-Sastre
Publication year - 2012
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.06384-11
Subject(s) - biology , virology , viral replication , recombinant dna , viral shedding , influenza a virus , virus , antiviral protein , interferon , orthomyxoviridae , viral protein , messenger rna , protein expression , rna , gene , genetics
Influenza A viruses containing the promoter mutations G3A/C8U in a given segment express increased levels of the corresponding viral protein during infection due to increased levels of mRNA or cRNA species. The replication of these recombinant viruses is attenuated, and they have an enhanced shedding of noninfectious particles and are incapable of antagonizing interferon (IFN) effectively. Our findings highlight the possibility of increasing influenza virus protein expression and the need for a delicate balance between influenza viral replication, protein expression, and assembly.
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