Open AccessExpression of Herpes Simplex Virus 1 MicroRNAs in Cell Culture Models of Quiescent and Latent Infection
Author(s) -
Igor Jurak,
Michael Hackenberg,
Ju Youn Kim,
Jean M. Pesola,
Roger D. Everett,
Chris M. Preston,
Angus C. Wilson,
Donald M. Coen
Publication year - 2013
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.03486-13
Subject(s) - biology , herpes simplex virus , microrna , virology , virus latency , virus , latent virus , cell culture , simplexvirus , latency (audio) , cell , herpesviridae , viral replication , in vivo , microbiology and biotechnology , gene , viral disease , genetics , electrical engineering , engineering
To facilitate studies of herpes simplex virus 1 latency, cell culture models of quiescent or latent infection have been developed. Using deep sequencing, we analyzed the expression of viral microRNAs (miRNAs) in two models employing human fibroblasts and one using rat neurons. In all cases, the expression patterns differed from that in productively infected cells, with the rat neuron pattern most closely resembling that found in latently infected human or mouse ganglia in vivo.
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