Open AccessAdenovirus Virus-Associated RNAs Induce Type I Interferon Expression through a RIG-I-Mediated Pathway
Author(s) -
Takeharu Minamitani,
Dai Iwakiri,
Kenzo Takada
Publication year - 2011
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.02160-10
Subject(s) - biology , rig i , interferon , innate immune system , virus , virology , interferon type i , rna , gene silencing , rna silencing , adenoviridae , adenovirus infection , interferon regulatory factors , gene expression , microbiology and biotechnology , immune system , rna interference , gene , genetic enhancement , immunology , genetics
The current study demonstrates that adenovirus virus-associated RNA (VA) is recognized by retinoic acid-inducible gene I (RIG-I), a cytosolic pattern recognition receptor, and activates RIG-I downstream signaling, leading to the induction of type I interferons (IFNs), similarly to Epstein-Barr virus-encoded small RNA. Further analysis revealed that adenovirus infection leads to biphasic type I IFN induction at 12 to 24 h and 48 to 60 h postinfection. The later induction coincided with VA expression and was reduced by virus UV inactivation or RIG-I silencing. These results suggest that VA-mediated RIG-I activation is involved in activating innate immune responses during adenovirus infection.