Open AccessA Hepatitis C Virus NS5A Phosphorylation Site That Regulates RNA Replication
Author(s) -
K. L. LeMay,
Jason Treadaway,
Ivan L. Ângulo,
T. Tellinghuisen
Publication year - 2012
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.02154-12
Subject(s) - ns5a , biology , rna , virology , rna dependent rna polymerase , replication factor c , viral replication , hyperphosphorylation , phosphorylation , hepatitis c virus , microbiology and biotechnology , genetics , virus , hepacivirus , control of chromosome duplication , dna replication , gene
The hepatitis C virus NS5A protein is essential for RNA replication and virion assembly. NS5A is phosphorylated on multiple residues during infections, but these sites remain uncharacterized. Here we identify serine 222 of genotype 2a NS5A as a phosphorylation site that functions as a negative regulator of RNA replication. This site is a component of the hyperphosphorylated form of NS5A, which is in good agreement with previous observations that hyperphosphorylation negatively affects replication.
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