Open AccessSimian Immunodeficiency Virus SIVmac239Δnef Vaccination Elicits Different Tat 28-35 SL8-Specific CD8 + T-Cell Clonotypes Compared to a DNA Prime/Adenovirus Type 5 Boost Regimen in Rhesus Macaques
Author(s) -
Benjamin J. Burwitz,
Zachary Ende,
Benjamin Sudolcan,
Matthew R. Reynolds,
Justin Greene,
Benjamin N. Bimber,
Jorge Reis Almeida,
Monica Kurniawan,
Vanessa Venturi,
Emma Gostick,
Roger W. Wiseman,
Daniel C. Douek,
David A. Price,
David H. O’Connor
Publication year - 2011
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.02112-10
Subject(s) - simian immunodeficiency virus , biology , virology , cd8 , cytotoxic t cell , t cell , t cell receptor , epitope , immunodeficiency , antigen , virus , immunology , immune system , genetics , in vitro
Different human immunodeficiency virus (HIV)/simian immunodeficiency virus (SIV) vaccine vectors expressing the same viral antigens can elicit disparate T-cell responses. Within this spectrum, replicating variable vaccines, like SIVmac239Δnef, appear to generate particularly efficacious CD8+ T-cell responses. Here, we sequenced T-cell receptor β-chain (TRB ) gene rearrangements from immunodominant Mamu-A*01-restricted Tat28-35 SL8-specific CD8+ T-cell populations together with the corresponding viral epitope in four rhesus macaques during acute SIVmac239Δnef infection. Ultradeep pyrosequencing showed that viral variants arose with identical kinetics in SIVmac239Δnef and pathogenic SIVmac239 infection. Furthermore, distinct Tat28-35 SL8-specific T-cell receptor (TCR) repertoires were elicited by SIVmac239Δnef compared to those observed following a DNA/Ad5 prime-boost regimen, likely reflecting differences in antigen sequence stability.