Open AccessRecombination-Dependent Oligomerization of Human Papillomavirus Genomes upon Transient DNA Replication
Author(s) -
Marit Orav,
Liisi Henno,
Helen Isok-Paas,
Jelizaveta Geimanen,
Mart Ustav,
Ene Ustav
Publication year - 2013
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.01798-13
Subject(s) - biology , recombination , dna replication , dna , genome , genetics , replication (statistics) , human papillomavirus , virology , homologous recombination , papillomaviridae , transient (computer programming) , microbiology and biotechnology , gene , cancer , cervical cancer , medicine , operating system , computer science
We describe the extensive and progressive oligomerization of human papillomavirus (HPV) genomes after transfection into the U2OS cell line. The HPV genomic oligomers are extrachromosomal concatemeric molecules containing the viral genome in a head-to-tail orientation. The process of oligomerization does not depend on the topology of the input DNA, and it does not require any other viral factors besides replication proteins E1 and E2. We provide evidence that oligomerization of the HPV18 and HPV11 genomes involves homologous recombination. We also demonstrate oligomerization of the HPV18 and HPV11 genomes in SiHa, HeLa, and C-33 A cell lines and provide examples of oligomeric HPV genomes in clinical samples obtained from HPV-infected patients.
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