Open AccessGag-Dependent Enrichment of HIV-1 RNA near the Uropod Membrane of Polarized T Cells
Author(s) -
Steven C. Hatch,
Luca Sardo,
Jianbo Chen,
Ryan C. Burdick,
Robert J. Gorelick,
Matthew J. Fivash,
Vinay K. Pathak,
Wei-Shau Hu
Publication year - 2013
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.01680-13
Subject(s) - biology , rna , microbiology and biotechnology , viral replication , human immunodeficiency virus (hiv) , cell , virus , virology , fluorescence microscope , cell membrane , biophysics , fluorescence , gene , biochemistry , physics , quantum mechanics
The enrichment of HIV-1 macromolecules at the uropod of polarized T cells can significantly promote virus assembly and cell-mediated infection. Using live-cell fluorescence microscopy, we demonstrate that full-length HIV-1 RNA is enriched at the uropod membrane; furthermore, the presence of HIV-1 Gag containing a functional nucleocapsid domain is necessary for this HIV-1 RNA enrichment. The results from these studies provide novel insights into the mechanism of HIV-1 replication in polarized T cells.
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