Open AccessHumanized NOD/SCID/IL2RγnullMice Transplanted with Hematopoietic Stem Cells under Nonmyeloablative Conditions Show Prolonged Life Spans and Allow Detailed Analysis of Human Immunodeficiency Virus Type 1 Pathogenesis
Author(s) -
Sumio Watanabe,
Shinrai Ohta,
Misako Yajima,
Kazuo Terashima,
Mamoru Ito,
Hideo Mugishima,
Shigeyoshi Fujiwara,
Kazufumi Shimizu,
Mitsuo Honda,
Norio Shimizu,
Naoki Yamamoto
Publication year - 2007
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.01353-07
Subject(s) - biology , nod , haematopoiesis , viremia , virology , severe combined immunodeficiency , stem cell , immunology , humanized mouse , immunodeficiency , hematopoietic stem cell transplantation , cxcr4 , virus , cancer research , microbiology and biotechnology , immune system , in vivo , genetics , chemokine
In a previous study, we demonstrated that humanized NOD/SCID/IL2Rγnull (hNOG) mice constructed with human hematopoietic stem cells (HSCs) allow efficient human immunodeficiency virus type 1 (HIV-1) infection. However, HIV-1 infection could be monitored for only 43 days in the animals due to their short life spans. By transplanting HSCs without any myeloablation methods, the mice successfully survived longer than 300 days with stable engraftment of human cells. The mice showed high viremia state for more than the 3 months examined, with systemic HIV-1 infection and gradual decrease of CD4+ T cells analogous to that in humans. These capacities of the hNOG mice are very attractive for modeling mechanisms of AIDS progression and therapeutic strategy.