Restricted Varicella-Zoster Virus Transcription in Human Trigeminal Ganglia Obtained Soon after Death | Zendy

Werner J. D. Ouwendijk | Zendy; Alexander Choe | Zendy; Maria A. Nagel | Zendy; Don Gilden | Zendy; Albert D. M. E. Osterhaus | Zendy; Randall J. Cohrs | Zendy; Georges M. G. M. Verjans | Zendy

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Restricted Varicella-Zoster Virus Transcription in Human Trigeminal Ganglia Obtained Soon after Death

Author(s) -

Werner J. D. Ouwendijk,

Alexander Choe,

Maria A. Nagel,

Don Gilden,

Albert D. M. E. Osterhaus,

Randall J. Cohrs,

Georges M. G. M. Verjans

Publication year - 2012

Publication title -

journal of virology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.617

H-Index - 292

eISSN - 1070-6321

pISSN - 0022-538X

DOI - 10.1128/jvi.01331-12

Subject(s) - varicella zoster virus , biology , reverse transcriptase , virology , virus , reverse transcription polymerase chain reaction , transcriptome , messenger rna , multiplex , transcription (linguistics) , polymerase chain reaction , microbiology and biotechnology , gene , gene expression , genetics , linguistics , philosophy

We analyzed the varicella-zoster virus (VZV) transcriptome in 43 latently infected human trigeminal ganglia (TG) with postmortem intervals (PMIs) ranging from 3.7 to 24 h. Multiplex reverse transcriptase PCR (RT-PCR) revealed no VZV transcripts with a PMI of <9 h. Real-time PCR indicated a significant increase (P = 0.02) in VZV ORF63 transcript levels but not the virus DNA burden with longer PMI. Overall, both the breadth of the VZV transcriptome and the VZV ORF63 transcript levels in human cadaver TG increased with longer PMI.

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