Open AccessPapillomavirus Particles Assembled in 293TT Cells Are Infectious In Vivo
Author(s) -
Timothy D. Culp,
Nancy M. Cladel,
Karla K. Balogh,
Lynn R. Budgeon,
Andrés Mejía,
Neil D. Christensen
Publication year - 2006
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.01328-06
Subject(s) - biology , virology , genome , heterologous , capsid , transfection , virus , in vivo , viral replication , papillomaviridae , cell culture , gene , genetics , cervical cancer , cancer
Papillomaviruses (PVs) demonstrate both tissue and species tropisms. Because PVs replicate only in terminally differentiating epithelium, the recent production of infectious PV particles in 293 cells marks an important breakthrough. In this article, we demonstrate that infectious PV particles produced in 293TT cells can cause papillomatous growths in the natural host animal. Moreover, we show that species-matched PV genomes can be successfully delivered in vivo by a heterologous, species-mismatched PV capsid. Additionally, our results indicate that the addition of the simian virus 40 origin of replication to the papillomavirus genome increases the production of infectious papillomavirus particles by increasing genome amplification in the transfected 293TT cells.