Open AccessSerum- and Glucocorticoid-Regulated Kinase 1 Is Required for Nuclear Export of the Ribonucleoprotein of Influenza A Virus
Author(s) -
Judith Alamares-Sapuay,
Luis MartínezGil,
Silke Stertz,
Matthew S. Miller,
Megan L. Shaw,
Peter Palese
Publication year - 2013
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.01258-12
Subject(s) - sgk1 , biology , ribonucleoprotein , nuclear export signal , virology , viral replication , small interfering rna , virus , influenza a virus , heterogeneous nuclear ribonucleoprotein , nuclear transport , kinase , viral interference , orthomyxoviridae , microbiology and biotechnology , rna , cell nucleus , gene , genetics , nucleus
We previously performed a small interfering RNA (siRNA) screen and identified serum- and glucocorticoid-regulated kinase 1 (SGK1) as a host factor required for influenza A virus replication. However, the role of SGK1 in the influenza viral life cycle has never been examined. In this study, we demonstrate that SGK1 is required for optimal replication of influenza virus, using the SGK1 inhibitor GSK 650394 and SGK1-specific siRNAs. We also demonstrate that SGK1 is required for viral ribonucleoprotein nuclear export.
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