Open AccessEfficient Reactivation of Latent Herpes Simplex Virus from Mouse Central Nervous System Tissues
Author(s) -
ShihHeng Chen,
Hui Yao,
Wen Huang,
Kuei Sen Hsu,
Huan Lei,
AiLi Shiau,
Shun Hua Chen
Publication year - 2006
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.01232-06
Subject(s) - biology , herpes simplex virus , virology , ex vivo , virus latency , central nervous system , virus , latent virus , herpesviridae , explant culture , in vivo , hsl and hsv , viral replication , in vitro , viral disease , genetics , neuroscience
For decades, numerous ex vivo studies have documented that latent herpes simplex virus (HSV) reactivates efficiently from ganglia, but rarely from the central nervous systems (CNS), of mice when assayed by mincing tissues before explant culture, despite the presence of viral genomes in both sites. Here we show that 88% of mouse brain stems reactivated latent virus when they were dissociated into cell suspensions before ex vivo explant culture. The efficient reactivation of HSV from the mouse CNS was demonstrated with more than one viral strain, viral serotype, and mouse strain, further indicating that the CNS can be an authentic latency site for HSV with the potential to cause recurrent disease.