Open AccessAssociation of Rice Gall Dwarf Virus with Microtubules Is Necessary for Viral Release from Cultured Insect Vector Cells
Author(s) -
Tàiyún Wèi,
Tamaki Uehara-Ichiki,
Naoyuki Miyazaki,
Hiroki Hibino,
Katsunori Iwasaki,
Toshihiro Omura
Publication year - 2009
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.01067-09
Subject(s) - biology , microtubule , virology , virus , viral replication , reoviridae , alfalfa mosaic virus , viral entry , microbiology and biotechnology , coat protein , rna , genetics , rotavirus , gene
Vector insect cells infected withRice gall dwarf virus , a member of the familyReoviridae , contained the virus-associated microtubules adjacent to the viroplasms, as revealed by transmission electron, electron tomographic, and confocal microscopy. The viroplasms, putative sites of viral replication, contained the nonstructural viral proteins Pns7 and Pns12, as well as core protein P5, of the virus. Microtubule-depolymerizing drugs suppressed the association of viral particles with microtubules and prevented the release of viruses from cells without significantly affecting viral multiplication. Thus, microtubules appear to mediate viral transport within and release of viruses from infected vector cells.