Human Cytomegalovirus UL84 Protein Contains Two Nuclear Export Signals and Shuttles between the Nucleus and the Cytoplasm | Zendy

Peter Lischka | Zendy; Claudia Rauh | Zendy; Regina Mueller | Zendy; Thomas Stamminger | Zendy

Open Access

Human Cytomegalovirus UL84 Protein Contains Two Nuclear Export Signals and Shuttles between the Nucleus and the Cytoplasm

Author(s) -

Peter Lischka,

Claudia Rauh,

Regina Mueller,

Thomas Stamminger

Publication year - 2006

Publication title -

journal of virology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.617

H-Index - 292

eISSN - 1070-6321

pISSN - 0022-538X

DOI - 10.1128/jvi.00995-06

Subject(s) - nuclear export signal , biology , nuclear localization sequence , nuclear transport , nuclear protein , cytoplasm , microbiology and biotechnology , nls , nucleus , cell nucleus , human cytomegalovirus , computational biology , genetics , virus , gene , transcription factor

Previous studies defined pUL84 of human cytomegalovirus as an essential regulatory protein with nuclear localization that was proposed to act during initiation of viral-DNA synthesis. Recently, we demonstrated that a complex domain of 282 amino acids within pUL84 functions as a nonconventional nuclear localization signal. Sequence inspection of this domain revealed the presence of motifs with homology to leucine-rich nuclear export signals. Here, we report the identification of two functional, autonomous nuclear export signals and show that pUL84 acts as a CRM-1-dependent nucleocytoplasmic shuttling protein. This suggests an unexpected cytoplasmic role for this essential viral regulatory protein.

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