Open AccessInfluence of pr-M Cleavage on the Heterogeneity of Extracellular Dengue Virus Particles
Author(s) -
Jiraphan Junjhon,
Tony Edwards,
Utaiwan Utaipat,
Valorie D. Bowman,
Heather A. Holdaway,
Wei Zhang,
Poonsook Keelapang,
Chunya Puttikhunt,
Rushika Perera,
Paul R. Chipman,
Watchara Kasinrerk,
Prida Malasit,
Richard Kühn,
Nopporn Sittisombut
Publication year - 2010
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.00696-10
Subject(s) - dengue virus , biology , cleavage (geology) , virus , extracellular , virology , viral replication , glycoprotein , flavivirus , dengue fever , immunoprecipitation , microvesicles , capsid , mutant , microbiology and biotechnology , biophysics , biochemistry , gene , paleontology , microrna , fracture (geology)
During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular particles, the partially mature particles, as the major prM-containing particles in a dengue serotype 2 virus. Changes in the proportion of viral particles in the pr-M junction mutants exhibiting altered levels of prM cleavage suggest that the partially mature particles may represent an intermediate subpopulation in the virus maturation pathway. These findings are consistent with a model suggesting the progressive mode of prM cleavage.