Open AccessPhage Display Selection of Cyclic Peptides That Inhibit Andes Virus Infection
Author(s) -
Pamela R. Hall,
Brian Hjelle,
Hadya Njus,
Chunyan Ye,
Virginie Bondu-Hawkins,
David C. Brown,
Kathleen A. Kilpatrick,
Richard S. Larson
Publication year - 2009
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.00606-09
Subject(s) - phage display , biology , peptide , virology , peptide library , in vitro , virus , glycoprotein , antibody , microbiology and biotechnology , epitope , peptide sequence , biochemistry , immunology , gene
Specific therapy is not available for hantavirus cardiopulmonary syndrome caused by Andes virus (ANDV). Peptides capable of blocking ANDV infection in vitro were identified using antibodies against ANDV surface glycoproteins Gn and Gc to competitively elute a cyclic nonapeptide-bearing phage display library from purified ANDV particles. Phage was examined for ANDV infection inhibition in vitro, and nonapeptides were synthesized based on the most-potent phage sequences. Three peptides showed levels of viral inhibition which were significantly increased by combination treatment with anti-Gn- and anti-Gc-targeting peptides. These peptides will be valuable tools for further development of both peptide and nonpeptide therapeutic agents.