Open AccessDendritic Cells Loaded with Tumor B Cells Elicit Broad Immunity against Murine Gammaherpesvirus 68 but Fail To Prevent Long-Term Latency
Author(s) -
Janet L. Weslow-Schmidt,
Fang Ye,
Stephanie S. Cush,
Kathleen A. Stuller,
Marcia A. Blackman,
Emilio Flaño
Publication year - 2010
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.00571-10
Subject(s) - biology , virus latency , immunity , immunology , dendritic cell , follicular dendritic cells , vaccination , latency (audio) , immune system , virology , lymph , virus , cancer research , viral replication , t cell , medicine , antigen presenting cell , pathology , electrical engineering , engineering
It is still unknown whether a noninfectious gammaherpesvirus vaccine is able to prevent or reduce virus persistence. This led us to use dendritic cells loaded with tumor B cells as a vaccine approach for the murine gammaherpesvirus 68 (γHV68) model of infection. Dendritic cells loaded with UV-irradiated latently infected tumor B cells induce broad, strong, and long-lasting immunity against γHV68. Dendritic cell vaccination prevents the enlargement of lymph nodes and severely limits acute infection and early latency but does not prevent γHV68 from establishing long-term latency. Our findings support the concept that attenuated viruses may be the best vaccine option for preventing gammaherpesvirus persistence.