Open AccessArtificial Recombination May Influence the Evolutionary Analysis of Newcastle Disease Virus
Author(s) -
Qing Song,
Yongtong Cao,
Qun Li,
Min Gu,
Lei Zhong,
Shunlin Hu,
Hongquan Wan,
Xiufan Liu
Publication year - 2011
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.00544-11
Subject(s) - newcastle disease , recombination , genbank , biology , virus , genome , genetics , virology , homologous recombination , gene
The recombination rate in Newcastle disease virus (NDV) was as high as 10% in RDP analysis with full-length NDV genome sequences available in GenBank. We found that two NDV strains, China/Guangxi09/2003 and NDV/03/018, previously reported as recombinants, failed to show any evidence of recombination upon complete genome resequencing. Furthermore, we were able to reproduce artificial recombination by amplification of the M gene in a mixed sample of strains LaSota and ZJ1. It appears that the recombination of NDV is not as common as has been reported. NDV sequences in GenBank should be analyzed with caution during bioinformatic analyses for natural recombination events.