Open AccessCardif-Mediated Signaling Controls the Initial Innate Response to Dengue Virus In Vivo
Author(s) -
Stuart T. Perry,
Tyler R. Prestwood,
Steven M. Lada,
Chris A. Benedict,
Sujan Shresta
Publication year - 2009
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.00365-09
Subject(s) - biology , dengue virus , dengue fever , interferon , in vivo , virology , innate immune system , interferon type i , viral replication , virus , innate lymphoid cell , rna , immunology , immune system , gene , genetics
The role of Cardif-dependent signaling in controlling dengue virus (DENV) infection and regulating type I interferon (IFN) production in vivo was examined in Cardif-deficient mice. DENV RNA levels were significantly elevated in both the serum and lymphoid tissues of Cardif−/− mice at early times compared to those in wild-type animals. Type I IFN production was delayed in these locales of Cardif−/− mice until 18 h postinfection, indicating that Cardif regulates the initial type I IFN response in lymphoid tissues. In contrast, DENV viral loads in nonlymphoid tissues were similar between Cardif−/− and wild-type mice. These results reveal that RNA helicase-mediated sensing acts as a first line of innate defense against DENV infection in vivo and functions in a tissue-dependent manner.